Laser Scanning Microscope Magnification And Resolution

Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope.

Laser scanning microscope magnification and resolution. Clsm combines high resolution optical imaging with depth selectivity which allows us to do optical sectioning. The laser scanning microscope uses a scanning design called beam scanning where the laser image path is scanned in a raster pattern on the surface of the sample. It uses a 670 nm red wavelength helium neon. This means that we can view visual sections of tiny structures that.

Capturing multiple two dimensional images at different depths in a sample enables the. The confocal laser scanning microscope. With confocal laser scanning microscopy clsm we can find out even more. This first generation instrument images corneal structures at 400 magnification and has a field of view of 400 400 µm when used with a 63 objective lens that has a numerical aperture of 0 9.

Effect of laser scanning speed on image resolution. Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size. In some cases specimens should be sampled at more than 2 3 times the highest information frequency to allow for the possibility that the highest frequency was misjudged. In the confocal laser scanning microscope the highest frequency to be sampled f is imposed by the optical system and for a particular resolution specification.